Ron asked: Are there statutes or regulations of any kind regarding what types of materials (such as lack of permanence) should be used in the marking of courses for organized rides? I have seen many course markings that fade away rather quickly, and others that seem to last for years.
Although bike events frequently use pavement markings to define a route, they are not legal.
Any roadway markings must comply with the statute concerning uniform traffic control devices.
s. 316.0745 – Uniform Signals and Devices
(3) All official traffic control signals or official traffic control devices purchased and installed in this state by any public body or official shall conform with the manual and specifications published by the Department of Transportation pursuant to subsection (2).
Clearly, such markings are intended to direct the movement of traffic (bicycles). I believe the intent of the following statute is to avoid confusion for drivers and prevent dangerous situations. That probably would not happen with the usual course markings used for cycling events.
s. 316.077 – Display of Unauthorized Signs, Signals or Markings
(1) No person shall place, maintain or display upon or in view of any highway any unauthorized sign, signal, marking or device which purports to be or is an imitation of or resembles an official traffic control device or railroad sign or signal, or which attempts to direct the movement of traffic, or which hides from view or interferes with the effectiveness of any official traffic control device or any railroad sign or signal.
I recommend checking with your local authorities or Bike/Ped Advisory Committee before placing pavement markings for the route.